Biomolecular Engineering MCQ

Q1. Which of the following is NOT a primary objective of biochemical engineering?

Correct Answer: A

Biochemical engineering aims to minimize waste, not maximize.

Q2. Which component is added in cell culture media for instant growth?

Correct Answer: C

Hormones stimulate rapid cell growth.

Q3. The tripeptide used as an artificial sweetener is:

Correct Answer: B

Aspartame is used as a sweetener.

Q4. Which technique is used for protein characterization?

Correct Answer: D

All listed techniques are used.

Q5. In batch bioprocessing, a limitation is:

Correct Answer: A

Maintaining conditions is harder in batch.

Q6. In an ideal chemostat, which parameters are constant?

Correct Answer: D

All these are maintained constant.

Q7. Which of these is the first successful genetically engineered product?

Correct Answer: B

Insulin was the first.

Q8. Fed-batch operation involves:

Correct Answer: A

Fed-batch adds nutrients intermittently.

Q9. Immobilized cells are used to:

Correct Answer: D

Used for efficient bioprocessing.

Q10. The Michaelis constant (Km) indicates:

Correct Answer: B

Km is substrate concentration at half Vmax.

Q11. Enzyme activity can be inhibited by:

Correct Answer: C

Both types reduce activity.

Q12. Gene cloning involves:

Correct Answer: D

Cloning steps.

Q13. The oxygen transfer rate is important in:

Correct Answer: A

Oxygen supply is critical in aerobic.

Q14. The lag phase in microbial growth represents:

Correct Answer: B

Cells adapt but don’t divide much.

Q15. Bioreactors control parameters like:

Correct Answer: D

All are controlled.

Q16. Which step follows substrate uptake in metabolism?

Correct Answer: B

Next step is energy-yielding.

Q17. Which bioprocessing technique allows continuous feed and removal?

Correct Answer: C

Continuous bioprocessing.

Q18. The enzyme turnover number is:

Correct Answer: A

Defines catalytic efficiency.

Q19. Which cell culture method is used for scale up?

Correct Answer: C

Microcarriers allow large-scale culture.

Q20. The product yield in a bioprocess is:

Correct Answer: A

Yield relates product to substrate.

Q21. Plasmids in genetic engineering are:

Correct Answer: B

Plasmids are circular DNA.

Q22. Which technique separates proteins by size and charge?

Correct Answer: A

SDS-PAGE separates by size and charge.

Q23. Which of the following is NOT a sterilization method?

Correct Answer: D

Filtration removes particles but not sterile.

Q24. Metabolic engineering involves:

Correct Answer: A

Optimizing metabolic routes.

Q25. The industrial use of enzymes depends on:

Correct Answer: D

All affect enzyme application.

Q26. Immobilization of enzymes is done to:

Correct Answer: D

Immobilization helps enzyme work.

Q27. Which bioreactor type uses airlift to mix contents?

Correct Answer: B

Airlift uses gas bubbles.

Q28. Which measure indicates specific growth rate of cells?

Correct Answer: C

Specific growth is rate of cell concentration change.

Q29. Cell disruption methods include:

Correct Answer: D

All methods used.

Q30. Which vitamin is commonly added in cell culture media?

Correct Answer: C

Vitamin B12 is essential.

Q31. Which is the main enzyme in DNA replication?

Correct Answer: B

DNA polymerase synthesizes DNA.

Q32. A bioprocess parameter monitored continuously is:

Correct Answer: D

All continuously monitored.

Q33. Which of the following is used for recombinant protein production?

Correct Answer: A

Fermentation produces proteins.

Q34. Which enzyme catalyzes the breakdown of starch?

Correct Answer: A

Amylase breaks starch.

Q35. Which parameter affects enzyme kinetics?

Correct Answer: D

All impact enzyme activity.

Q36. Gene cloning uses which molecule as a vector?

Correct Answer: D

Vectors include plasmids, phages, etc.

Q37. Which step involves purification of bioproduct?

Correct Answer: B

Downstream includes purification.

Q38. Which is NOT typically part of downstream processing?

Correct Answer: D

Fermentation is upstream.

Q39. The term β€œspecific activity” of enzyme refers to:

Correct Answer: B

Activity per unit protein.

Q40. Which method helps maintain sterile conditions during bioprocessing?

Correct Answer: D

All help achieve sterility.

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