Biomedical Optics MCQ

Q1. What is the refractive index?

Correct Answer: A

Refractive index defines bending of light when entering a medium.

Q2. Which of the following are fundamental optical properties of biological tissues?

Correct Answer: A

Main optical properties are absorption, scattering, anisotropy.

Q3. Which physics theory explains light as discrete photons?

Correct Answer: B

Quantum theory explains photons.

Q4. What is the meaning of scattering coefficient in biomedical optics?

Correct Answer: A

Scattering coefficient quantifies scattering per distance.

Q5. Total internal reflection occurs when light passes from:

Correct Answer: A

Occurs when wave moves from denser to rarer medium.

Q6. Which optical method is used for fluorophore detection in tissues?

Correct Answer: B

Fluorescence spectroscopy detects fluorophores.

Q7. The Beer-Lambert law relates:

Correct Answer: B

Describes attenuation due to absorption.

Q8. Which part of the optical spectrum is used in Near-Infrared Spectroscopy (NIRS)?

Correct Answer: C

NIRS uses 700–900 nm range.

Q9. Which optical imaging technique provides high-resolution cross-sectional images of biological tissue?

Correct Answer: B

OCT produces cross-sectional images.

Q10. In laser tissue interaction, β€œphotothermal effect” is:

Correct Answer: A

Photothermal effect converts light to heat.

Q11. Which type of light source is most commonly used in biomedical optics for fluorescence excitation?

Correct Answer: B

Lasers provide high intensity and monochromatic light.

Q12. Mie scattering occurs when scatterer size is:

Correct Answer: B

Mie scattering dominates when size is comparable to wavelength.

Q13. What is the main clinical application of photoacoustic imaging?

Correct Answer: A

Photoacoustic imaging maps blood oxygen.

Q14. Coherent light used in optical imaging means:

Correct Answer: A

Coherent light waves have fixed phase.

Q15. Which parameter determines the resolution in optical microscopy?

Correct Answer: A

Numerical aperture affects resolution.

Q16. In biomedical optics, scattering anisotropy describes:

Correct Answer: B

Anisotropy refers to scattering directionality.

Q17. Laser therapy used in medicine primarily employs:

Correct Answer: B

Laser converts light energy to heat.

Q18. In optical fiber sensors, signal transmission occurs via:

Correct Answer: B

Light signals transmit in optical fibers.

Q19. Autofluorescence in tissues is due to:

Correct Answer: B

Intrinsic molecules fluoresce naturally.

Q20. Photodynamic therapy involves:

Correct Answer: A

Photosensitizers activated by light.

Q21. Which optical parameter depends on tissue composition?

Correct Answer: D

Scattering coefficient varies by tissue.

Q22. Infrared radiation in biomedical optics is used primarily for:

Correct Answer: A

Infrared detects thermal variations.

Q23. Which method uses multiple photons to excite fluorescence in tissues?

Correct Answer: B

Multiphoton excitation involves multiple photons.

Q24. Which parameter uniquely defines light absorption?

Correct Answer: A

Absorption coefficient quantifies light absorbed.

Q25. The Soret band is associated with:

Correct Answer: B

Soret band arises due to heme absorption.

Q26. Which method enhances optical image resolution beyond diffraction:

Correct Answer: B

STED allows super-resolution imaging.

Q27. Which tissue component causes light scattering at cellular level?

Correct Answer: A

Nuclei are major scatterers of light.

Q28. What does the Fresnel reflection describe?

Correct Answer: A

Reflection off surfaces with refractive index contrast.

Q29. The term "therapeutic window" in biomedical optics refers to:

Correct Answer: B

Wavelengths where tissues absorb light safely/effectively.

Q30. Optical coherence tomography (OCT) is based on:

Correct Answer: B

OCT uses low coherence interferometry.

Q31. Which parameter quantifies scattering mean free path?

Correct Answer: A

Mean free path = 1 / scattering coefficient.

Q32. Which laser type is commonly used in biomedical optics?

Correct Answer: D

Various types used in medicine.

Q33. Which optical technique is used for measuring oxygenation in tissue?

Correct Answer: A

Near-infrared light measures oxygenation.

Q34. Which biological molecule is highly fluorescent under UV light?

Correct Answer: D

Proteins with tryptophan, tyrosine fluoresce.

Q35. Which optical method is label-free?

Correct Answer: B

Raman measures molecular vibrations without labels.

Q36. Ophthalmoscopy is a diagnostic method for:

Correct Answer: B

Ophthalmoscope examines the retina.

Q37. Which parameter defines how light intensity decays in tissue?

Correct Answer: C

Attenuation includes absorption and scattering.

Q38. Which instrument is used to measure the refractive index?

Correct Answer: B

Refractometer measures refractive index.

Q39. Light scattering in tissues is mainly influenced by:

Correct Answer: B

Scattering depends on microscopic structure.

Q40. What is the key property of lasers used in medicine?

Correct Answer: A

Coherence allows precision.

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